Description
NAPzol is a ready-to-use reagent designed to isolate high-quality total RNA from human, animal, plant, yeast, or bacterial cell and tissue samples within one hour. This monophasic solution contains phenol, guanidine salts, and proprietary components that facilitate the isolation of various RNA species, including both large and small molecules. NAPzol preserves RNA integrity by effectively inhibiting RNase activity while disrupting cells and dissolving cell components during homogenization. It allows for the simultaneous processing of multiple samples and enhances the single-step RNA isolation method.
The NAPzol reagent enables users to isolate RNA, DNA, and proteins sequentially from a single sample. After homogenizing the sample with NAPzol, chloroform is added. The mixture then separates into three layers: a clear upper aqueous layer, an interphase, and a lower red organic layer. RNA is precipitated from the aqueous layer using isopropanol, while DNA is precipitated from the interphase/organic layer using ethanol. Proteins are precipitated from the phenol-ethanol supernatant using isopropanol. The precipitated RNA, DNA, or protein is then washed to remove impurities and resuspended for downstream applications.